Catalogue
Browse the RNA and reagent products offered by the RNA and Formulation Core.
Low dsRNA T7 Polymerase (UBC Only)
A mutant T7 polymerase designed for low dsRNA IVT products. Typically drop-in replacable for WT T7 reactions. Due to reagent designs, this is currently not directly compatible with our IVT kits.
BspQI-HF (UBC Only)
Access our volume pricing for NEB's BspQI-HF restriction enzyme.
IVT Kit 1 mg (UBC Only)
This kit contains a master mix of ATP, CTP, TTP, and N1-methylpseudoUTP, GAG Cap1 trinucleotide co-capping reagent, WT T7 polymerase, pyrophosphatase, RNase inhibitor, and buffers for IVT synthesis. Widely compatible with most GAG co-capping templates. Up to 1.2 mg yield can be achieved, depending on nucleotide usage. Please contact us for a shared protocol or questions about its use.
IVT Kit 10 x 1 mg (UBC Only)
This kit contains a master mix of ATP, CTP, TTP, and N1-methylpseudoUTP, GAG Cap1 trinucleotide co-capping reagent, WT T7 polymerase, pyrophosphatase, RNase inhibitor, and buffers for IVT synthesis. Widely compatible with most GAG co-capping templates. Up to 12 mg yield can be achieved, depending on nucleotide usage. Please contact us for a shared protocol or questions about its use.
Fluc
Encodes the Firefly luciferase gene, providing a convenient bioluminescent marker, flanked by optimized human β-globin 5' and 3' UTRs. Contains Cap1, complete subtitution of UTP with N1-methylpseudoUTP during synthesis, and a 120 long poly(A) tail. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography.
Nluc
Encodes Promega's licensed Nano luciferase gene, providing a small, 100-fold brighter bioluminescent marker than standard luciferases, flanked by optimized human β-globin 5' and 3' UTRs. Requires coelenterazine as a substrate. Contains Cap1, complete subtitution of UTP with N1-methylpseudoUTP during synthesis, and a 120 long poly(A) tail. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography. Recepients external to UBC must have a current MSA with the limited use license terms and conditions outlined in the MSA.
EGFP
Encodes the enhanced green fluorescent protein gene, providing a convenient fluorescent marker, flanked by optimized human β-globin 5' and 3' UTRs. Contains Cap1, complete subtitution of UTP with N1-methylpseudoUTP during synthesis, and a 120 long poly(A) tail. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography.
Cy5 EGFP
Encodes the enhanced green fluorescent protein gene, providing a convenient fluorescent marker, flanked by optimized human β-globin 5' and 3' UTRs. Contains Cap1, complete subtitution of UTP with 80% N1-methylpseudoUTP and 20% Cy5-UTP during synthesis, and a 120 long poly(A) tail. The Cy5 dye provide particle tracking possibilities; however, reduced EGFP expression can be observed. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography.
Ovalbumin
Encodes the Ovalbumin gene, regularly used as an antigen for immune studies, flanked by optimized human β-globin 5' and 3' UTRs. Contains Cap1, complete subtitution of UTP with N1-methylpseudoUTP during synthesis, and a 120 long poly(A) tail. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography.
secNluc
Encodes a secreted version of Promega's licensed Nano luciferase gene, providing a small, 100-fold brighter bioluminescent marker than standard luciferases, flanked by optimized human β-globin 5' and 3' UTRs. Requires coelenterazine as a substrate. Contains Cap1, complete subtitution of UTP with N1-methylpseudoUTP during synthesis, and a 120 long poly(A) tail. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography. Recepients external to UBC must have a current MSA with the limited use license terms and conditions outlined in the MSA.
NlucP
Encodes a destabilized version of Promega's licensed Nano luciferase gene, providing a rapidly degraded, 100-fold brighter bioluminescent marker than standard luciferases, flanked by optimized human β-globin 5' and 3' UTRs. Requires coelenterazine as a substrate. With a shorter half life, luminescence is more decoupled from protein stability for improved RNA half-life assays. Contains Cap1, complete subtitution of UTP with N1-methylpseudoUTP during synthesis, and a 120 long poly(A) tail. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography. Recepients external to UBC must have a current MSA with the limited use license terms and conditions outlined in the MSA.
mCherry
Encodes the mCherry gene, providing a convenient fluorescent marker, flanked by optimized human β-globin 5' and 3' UTRs. Contains Cap1, complete subtitution of UTP with N1-methylpseudoUTP during synthesis, and a 120 long poly(A) tail. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography.
SARS-CoV-2 Spike (Wuhan)
Encodes the SARS-CoV-2 Spike (Wuhan Variant) gene, regularly used as an antigen for immune studies, flanked by optimized human β-globin 5' and 3' UTRs. Contains Cap1, complete subtitution of UTP with N1-methylpseudoUTP during synthesis, and a 120 long poly(A) tail. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography.
Cas9
Encodes the Cas9 gene, regularly used in CRISPR/Cas9 studies, flanked by optimized human β-globin 5' and 3' UTRs. Contains Cap1, complete subtitution of UTP with N1-methylpseudoUTP during synthesis, and a 120 long poly(A) tail. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography.
mScarlet3 (UBC Only)
Encodes the mScarlet3 gene, providing a bright red fluorescent marker, flanked by optimized human β-globin 5' and 3' UTRs. Contains Cap1, complete subtitution of UTP with N1-methylpseudoUTP during synthesis, and a 120 long poly(A) tail. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography. Sold as an internal research product only due to IP.
RNA Storage Solution (UBC Only)
A slightly acidic, low salt pH buffer for improved RNA stability. Does not contain EDTA.
Fluc
Encodes the Firefly luciferase gene, providing a convenient bioluminescent marker, within a replication cassette of the TC83 strain of VEEV . Contains Cap1, canonical nucletides and an approximately 100 long poly(A) tail. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography.
Gluc
Encodes the Gaussia luciferase gene, providing a convenient and naturally secreted bioluminescent marker, within a replication cassette of the TC83 strain of VEEV . Contains Cap1, canonical nucletides and an approximately 100 long poly(A) tail. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography.
Nluc
Encodes Promega's licensed Nano luciferase gene, providing a small, 100-fold brighter bioluminescent marker within a replication cassette of the TC83 strain of VEEV. Requires coelenterazine as a substrate. Contains Cap1, canonical nucletides and an approximately 100 long poly(A) tail. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography. Recepients external to UBC must have a current MSA with the limited use license terms and conditions outlined in the MSA.
EGFP
Encodes the enhanced green fluorescent protein gene, providing a convenient fluorescent marker, within a replication cassette of the TC83 strain of VEEV . Contains Cap1, canonical nucletides and an approximately 100 long poly(A) tail. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography.
Ovalbumin
Encodes the Ovalbumin gene, regularly used as an antigen for immune studies, within a replication cassette of the TC83 strain of VEEV . Contains Cap1, canonical nucletides and an approximately 100 long poly(A) tail. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography.
mCherry
Encodes the mCherry gene, providing a convenient fluorescent marker, within a replication cassette of the TC83 strain of VEEV . Contains Cap1, canonical nucletides and an approximately 100 long poly(A) tail. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography.
SARS-CoV-2 Spike (Wuhan)
Encodes the SARS-CoV-2 Spike (Wuhan Variant) gene, regularly used as an antigen for immune studies, within a replication cassette of the TC83 strain of VEEV . Contains Cap1, canonical nucletides and an approximately 100 long poly(A) tail. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography.
mScarlet3 (UBC Only)
Encodes the mScarlet3 gene, providing a convenient fluorescent marker, within a replication cassette of the TC83 strain of VEEV. Contains Cap1, canonical nucletides and an approximately 100 long poly(A) tail. Prepared using a mutant RNA polymerase for low dsRNA content and purified using oligo(dT) chromatography. Sold as an internal research product only due to IP.
Unlisted Product
We can offer access to a wide range of reagents through custom requests. Please enter the description of your needs in the final comments section during the request confirmation stage of this order.
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